Source: Guidelines for the quality-secured production of laboratory animal diets
Society for Laboratory Animal Science, GV-SOLAS, Committee for the Nutrition of Laboratory Animals, August 2001
Determined level |
Maximum deviation |
||
| Crude protein | < 5% 5 up to 10% > 10% |
-1a -10r -2a |
+1.5a +15r +3a |
| Crude fat | < 5% 5 up to 10% > 10% |
-0.8a -10r -1.5a |
+1.2a |
| Crude fibre | < 6% 6 up to 10% > 10% |
-1.4a -22.5r -2.7a |
+0.9a |
| Crude ash | < 5% 5 up to 10% > 10% |
-1.5a |
+0.5a +10r +1a |
| Water | < 5% 5 up to 10% > 10% |
--- |
+0.5a +10r +1a |
| Calcium, Phosphorus | <1% 1 up to 6% > 6% |
-0.15a -15r -0.9a |
+0.3a +22.5r +1.4a |
| Potassium, Sodium, Magnesium | < 0.7% 0.7 up to 5% |
-0.1a -15r |
+0.15a +22.5r |
| Lysine, Methionine, Threonine | -15r |
||
| Cystine, Tryptophan | -20r |
||
a: absolute deviation in % of the given value
r: relative deviation in % of the given value
Chemical methods
Crude protein VDLUFA, Vol. III, 4.1.1, v.19
Crude fat HCl-hydrolysis / EG method
Crude fiber VDLUFA method
Crude ash VDLUFA, Vol. III, 8.1, 1975-11
Calcium/Phosphorus Gravimetric
ADDITIVES IN LABORATORY ANIMAL FEEDING STUFF
| up to 0.5 units | mg, 1.000 mcg, 1.000 IE |
+- 40 | r |
| more than 0.5 units up to 1.0 units | mg, 1.000 mcg, 1.000 IE |
+- 0.2 units | a |
| more than 1.0 units up to 50 units | mg, 1.000 mcg, 1.000 IE |
+- 20 | r |
| more than 50 units up to 100 units | mg, 1.000 mcg, 1.000 IE |
+- 10 units | a |
| more than 100 units up to 500 units | mg, 1.000 mcg, 1.000 IE |
+- 10 | r |
| more than 500 units up to 1.000 units | mg, 1.000 mcg, 1.000 IE |
+- 50 units | a |
| more than 1.000 units | mg, 1.000 mcg, 1.000 IE |
+- 5 | r |
a: absolute deviation in % of the given level
r: relative deviation in % of the given level
MICROBIOLOGICAL GUIDE AND CRITICAL VALUES
(according to the recommendations of GV-SOLAS)
Guide value [cfu/g] |
Critical value [cfu/g] |
|
| Aerobic total germ count §64 LFGB 06.00-18* |
<1x10³ |
<1x105 |
| Yeasts / Moulds §64 LFGB 01.00-37* |
<200 |
<1x10³ |
| Enterobacteriaceae §64 LFGB 05.00-5* |
<100 E.coli <10 |
100 10 |
| Coagulase-positive staphylococci §64 LFGB 00.00-55* |
<10 |
10 |
| Salmonella §64 LFGB 00.00-20* |
not detected n in 25g |
--- |
* official method
cfu: colony forming unit
Detection of contaminants
Contents of undesired substances and contaminants in standard diets will be analysed two times a year.
Contaminants:
Aflatoxins B1, B2, G1, G2
Chlorinates pesticides
Heavy metals, fluorine and selenium
Organic chlorinated pesticides
Organic phosphorus pesticides
Pyrethroides
PCBs
Critical values of contaminants (GV-SOLAS)
Above-mentioned values refer to feeding stuff with 88% dry matter.
